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O-Glycosidically linked oligosaccharides from peptidorhamnomannans ofSporothrix schenckii
Authors:Leila M Lopes-Alves  Lucia Mendonça-Previato  Bernard Fournet  Pierre Degand  José O Previato
Institution:(1) Depart. Biologia Celular e Genética-UERJ, Rio de Janeiro, Brasil;(2) Depart. Microbiologia Geral, Inst. Microbiologia-CCS, Bl. I, Universidade Federal do Rio de Janeiro, 21941, Brasil;(3) Université des Sciences et Techniques de Lille-Flandres-Artois, 59.655, France;(4) Unité 16, INSERM, Place de Verdun, Lille, France
Abstract:beta-Elimination of peptidorhamnomannans purified from yeast-like and mycelial phases ofSporothrix schenckii released neutral and acidic reduced oligosaccharides that were O linked to serine and/or threonine. Man-(agr1–2)Man-ol, Rha(agr1–3)Man(agr1–2)Man-ol, Rha(agr1–4)GlcA(agr1–2)Man(agr1–2)Man-ol, and Rha(agr1–4)Rha(agr1–2)] GlcA(agr1–2)Man(agr1–2)Man-ol were characterized based on methylation analysis, proton magnetic resonance and fast atom bombardment mass spectrometry.Abbreviations FAB fast atom bombardment - GLC gas liquid chromatography - GlcA d-glucopyranosyluronic acid - Man d-mannopyranose - Man-ol d-mannitol - MS mass spectrometry - NMR nuclear magnetic resonance - Rha l-rhamnopyranose
Keywords:Oligosaccharides  Sporothrix schenckii
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