Effects of fumarate,l-malate,and anAspergillus oryzae fermentation extract ond-lactate Utilization by the ruminal bacteriumSelenomonas ruminantium |
| |
| Authors: | David J Nisbet Scott A Martin |
| |
| Institution: | (1) Department of Animal and Dairy Science, The University of Georgia, 308 Livestock-Poultry Building, 30602 Athens, Georgia, USA;(2) Department of Microbiology, The University of Georgia, 308 Livestock-Poultry Building, 30602 Athens, Georgia, USA;(3) Present address: Southern Plains Area Food Animal Protection Laboratory, USDA-ARS, 77845 College Station, TX, USA |
| |
| Abstract: | Growth ofSelenomonas ruminantium HD4 in medium that contained 21mm d-lactate was stimulated to varying degrees by 10mm l-malate, 10mm fumarate, and 2% (v/v)Aspergillus oryzae fermentation extract (Amaferm). Amaferm treatment caused the greatest growth stimulation. Initial uptake rates (30s) and long-term uptake rates (30 min) ofd-lactate by whole cells ofS. ruminantium were increased in the presence of 10mm l-malate. Amaferm (25  l/ml) also stimulated long-term uptake rates ofd-lactate, whereas fumarate had no effect. Initial uptake ofd-lactate was depressed in the presence of fumarate or Amaferm. When eitherl-malate, fumarate, or Amaferm was included in thed-lactate growth medium, a homosuccinate fermentation resulted and an inverse relationship was observed between growth (protein synthesis) and succinate production. Recent research demonstrated that Amaferm containsl-malate, and this dicarboxylic acid may be involved in stimulatingd-lactate utilization byS. ruminantium. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
