<Emphasis Type="Italic">In vitro</Emphasis> and <Emphasis Type="Italic">in vivo</Emphasis> inhibition of α-amylases of stored-product mite <Emphasis Type="Italic">Acarus siro</Emphasis> |
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Authors: | Jan?Hubert Lucie?Dole?ková-Mare?ová Jana?Hyblová Iva?Kudlíková Václav?Stejskal Email author" target="_blank">Michael?Mare?Email author |
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Institution: | (1) Research Institute of Crop Production, Drnovska 506, 16106 Praha, Czech Republic;(2) Institute of Organic Chemistry and Biochemistry, Flemingovo nam. 2, 16610 Praha, Czech Republic;(3) Czech University of Agriculture Prague, Kamycka 129, 16521, Czech Republic |
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Abstract: | The stored-product mites are the most abundant and frequent group of pests living on the stored food products in Europe. They endanger public health since they produce allergens and transmit mycotoxin-producing fungi. Novel acaricidal compounds with inhibitory effects on the digestive enzymes of arthropods are a safe alternative to the traditional neurotoxic pesticides used for control of the stored-product pests. In this work, we explored the properties of acarbose, the low molecular weight inhibitor of -amylases (AI), as a novel acaricide candidate for protection of the stored products from infestation by Acarus siro (Acari: Acaridae). In vitro analysis revealed that AI blocked efficiently the enzymatic activity of digestive amylases of A. siro, and decreased the physiological capacity of mites gut in utilizing a starch component of grain flour. In vivo experiments showed that AI suppressed the population growth of A. siro. The mites were kept for three weeks on experimental diet enriched by AI in concentration range of 0.005 to 0.25%. Population growth of A. siro was negatively correlated with the content of AI in the treated diet with a half-population dose of 0.125%. The suppressive effect of AIs on stored-product mites is discussed in the context of their potential application in GMO crops |
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Keywords: | -amylase" target="_blank">gif" alt="agr" align="BASELINE" BORDER="0">-amylase Acarus siro Inhibitor Stored-product mite |
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