A synthetic intron in a naturally intronless yeast pre-tRNA is spliced efficiently in vivo. |
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| Authors: | M Winey I Edelman and M R Culbertson |
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| Institution: | Laboratory of Genetics, University of Wisconsin, Madison 53706. |
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| Abstract: | Saccharomyces cerevisiae glutamine tRNA(CAG) is encoded by an intronless, single-copy gene, SUP60. We have imposed a requirement for splicing in the biosynthesis of this tRNA by inserting a synthetic intron in the SUP60 gene. Genetic analysis demonstrated that the interrupted gene produces a functional, mature tRNA product in vivo. |
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