藏红花瓣状体的诱导与花柱-柱头状物的定向分化

藏红花 (ＣｒｏｃｕｓｓａｔｉｖｕｓＬ .)又名番红花、西红花 ;藏药中称为苟日苟木。它是鸢尾科草本植物 ,原产于欧洲、地中海地区。我国长期以来把它作为珍贵的中藏药。其药用部位是柱头 ,有效成分主要是藏红花素、藏红花酸、藏红花醛和藏红花苦素。丁葆祖等于 1979年首次从藏红花的球茎获得完整的植株1 ] 。Ｓａｎｏ等人 1987年在离体条件下 ,诱导花柱 柱头状物再生获得成功2 ] ,此后国内外不断有相关报道3～ 7] 。但试验大多集中在由外植体直接或由愈伤组织间接诱导柱头状物 ,且花柱 柱头状物的频率低和数量少。我们试图先由藏红花…

Induction of Petal-like Structures from Petals of Crocus sativus L. and the Differentiation of Style-Stigma-like Structures in vitro

Firstly the petal of Crocus sativus L was cultured on the medium that supplemented with different combinations of hormones. The petal-like structures(PLS) were induced on medium, but the induction rates were different in various medium. The highest induction rate of petal-like structures was obtained on the media that was supplemented with NAA (4 mg/L) and KT (8 mg/L). The petal-like structures were subcultured on another media when the structure was produced on the explants and proliferate groups. The later media was used for inducing style-stigma-like structures(SSLS). The induction rate of style-stigma-like-structures in the petal-like structures group is much higher than the rate in the preceding work, and the maximum of style-stigma-like structures produced per explant was 30. The best result of style-stigma-like structures was observed on the petal-like structure groups which came from the third treatment. The differentiation rate of style-stigma-like structures is stable in the subcultures of petal-like structures. The result revealed that the induction frequency of style-stigma-like structures formed on the petal-like structures is higher than that form on the petals of C.sativus L.