| 鳜鱼传染性脾肾坏死病毒(ISKNV)PCR检测方法的建立及虹彩病毒新证据 |
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| 引用本文： | 邓敏,何建国,左涛,翁少萍,曾慷,吕玲,周松裕,龙綮新. 鳜鱼传染性脾肾坏死病毒(ISKNV)PCR检测方法的建立及虹彩病毒新证据[J]. 病毒学报, 2000, 16(4): 365-369 |
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| 作者姓名： | 邓敏 何建国 左涛 翁少萍 曾慷 吕玲 周松裕 龙綮新 |
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| 作者单位： | 中山大学,生命科学学院,广东,广州,510275;中山大学,生物防治国家重l点实验室,广东,广州,510275 |
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| 摘 要： | 利用真鲷虹彩病毒（ＲＳＩＶ）核苷酸还原酶小亚单位（ＲＮＲＳ）基因保守区设计的一对引物,建立了鳜鱼传染性脾肾坏死病毒（ＩＳＫＮＶ）特异的ＰＣＲ检测体系。运用该体系检测ＩＳＫＮＶ,具有简便、快速、敏感、特异等特点,为诊断与预防ＩＳＫＮＶ提供了一个重要的手段。通过对ＰＣＲ产物的克隆与序列分析,发现ＩＳＫＮＶ ＰＣＲ扩增产物与ＲＳＩＶ ＲＮＲＳ基因相应序列的同源性很高,达到９２．５％,进一步证明ＩＳＫＮＶ
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| 关 键 词： | 传染性脾肾坏死病毒 聚合酶链反应 检测 虹彩病毒 |
Infectious Spleen and Kidney Necrosis Virus (ISKNV) from Siniperca Chuatsi: Development of a PCR Detection Method and the New Evidence of Iridovirus |
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| DENG Min,HE Jian-guo,ZUO Tao,WENG Shao-ping,ZENG Kang,LU Ling,ZHOU Song-yu,LONG Qing-xin. Infectious Spleen and Kidney Necrosis Virus (ISKNV) from Siniperca Chuatsi: Development of a PCR Detection Method and the New Evidence of Iridovirus[J]. Chinese journal of virology, 2000, 16(4): 365-369 |
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| Authors: | DENG Min HE Jian-guo ZUO Tao WENG Shao-ping ZENG Kang LU Ling ZHOU Song-yu LONG Qing-xin |
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| Abstract: | A PCR based detection procedure for infectious spleen and kidney necrosis virus (ISKNV) from Siniperca chuatsi was developed by using a pair of primers which were designed from the sequence of the ribonucleotide reductase small subunit (RNRS) gene of red sea bream iridovirus (RSIV). The clone and sequence analysis of the PCR product confirmed that ISKNV is a iridovirus. |
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| Keywords: | infectious spleen and kidney necrosis virus PCR detection iridovirus |
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