Properties of solubilized prostaglandin synthetase from sheep vesicular glands. |
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Authors: | A Raz Y Kloog E Perel R Kenig-Wakshal |
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Institution: | Department of Biochemistry., The George S. Wise Center of Life Sciences Tel-Aviv University., Ramat-Aviv., Tel-Aviv, Israel. |
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Abstract: | Prostaglandin synthetase activity associated with the microsomal fraction from sheep vesicular glands has been solubilized by treatment with the non-ionic detergents Tween 20, Lubrol Px and Lubrol Wx. Approx.8 fold purification from microsomes is obtained and over 90% of the activity is recovered in the detergent solubilized fraction. The solubilized synthetase activity is stable at pH 5.0 but is gradually lost at pH 8.0; it is also heat and acid labile. The relative amounts of prostaglandins E2, D2 and F2α formed by the microsomal-bound synthetase and by the solubilized synthetase are similar. Also similar are the pH optima (7.9–8.5) of the two synthetase preparations. The solubilization process appears to yield a fully active enzymatic preparation which could be employed for further purification and characterization of the prostaglandin synthetase complex. |
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