柞蚕蛹虫草代谢物组分分析及对高尿酸血症的作用

采用定量检测和代谢组学方法对柞蚕蛹虫草的成分和参与的代谢途径及通路进行了分析,并开展了其对嘌呤代谢障碍引起的高尿酸血症作用研究。结果表明,柞蚕蛹虫草共含有代谢产物233种,其中不仅含有定量检测已经报道的虫草素、虫草酸等成分,还含有皮质固醇、肾上腺素及谷胱甘肽等多种特殊代谢成分,这些成分主要隶属于羧酸及衍生物、有机氧化合物及脂肪酰基类等10个不同类别,KEGG分析其显著性富集在ABC转运蛋白、氨基酸生物合成及嘌呤代谢等19种代谢途径;动物试验结果表明,柞蚕蛹虫草中、高剂量组与模型组比较,血尿酸水平和黄嘌呤氧化酶活性均显著降低（P<0.05）,对防治高尿酸血症具有明显的作用,并对其作用机制进行了分析探讨。本研究为柞蚕蛹虫草在高尿酸血症作用及其相关药理学研究和开发应用提供了理论依据。

Analysis of metabolite components of Cordyceps militaris and their effects on hyperuricemia

The components and involved metabolic pathways of Cordyceps militaris were analyzed by quantitative and metabonomics methods and their effects on hyperuricemia caused by purine metabolic disorders were studied.The results show that C.militaris contains 233 metabolites,including cordycepin and cordyceps acid already reported and corticosteroids,epinephrine and glutathione,etc.belonging to ten different categories such as carboxylic acids and their derivatives,organic oxides and fatty acyl groups.KEGG analysis showed their significant enrichment in 19 metabolic pathways including ABC transport protein,amino acid biosynthesis and purine metabolism.Compared with the experimental model group,the serum uric acid level and xanthine oxidase activities were significantly decreased in the middle and high dose treatment groups of mice administrated with C.militaris(P<0.05),showing obvious effects on the prevention and treatment of hyperuricemia.The functional mechanism of C.militaris metabolites was analyzed and discussed.