A study on the effects of some laboratory-derived genetic mutations on biofilm formation by Listeria monocytogenes |
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| Authors: | Kumar Sanath Parvathi Ammini George Jeffy Krohne Georg Karunasagar Indrani Karunasagar Iddya |
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| Institution: | (1) Department of Fishery Microbiology, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Mangalore, 575 002, India;(2) Molecular Biology Laboratory, Department of Biological Oceanography, National Institute of Oceanography (CSIR), Kochi, 682 018, India;(3) Division of Electron Microscopy, Biocenter of the University of Wurzburg, Am Hubland, 97074 Wurzburg, Germany;(4) ENMU 2386, 1500 S, Ave. K, Portales, NM 88130, USA |
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| Abstract: | Biofilms formed by the human pathogen Listeria monocytogenes in food-processing environments can be a potential source of contamination. In this study, we investigated the ability of
L. monocytogenes wild type and its laboratory-derived isogenic mutants in cwhA, prfA, agrA, flaA, degU, ami and sigB to adhere to and form biofilms on abiotic surfaces. The results suggest that inactivation of the two component regulatory
system degU completely abolished biofilm formation, while inactivation of the flagellar gene flaA, two component response regulator agrA and the autolysin-adhesin gene ami lead to severe impairment of initial attachment and the subsequent development of a mature biofilm by L. monocytogenes. Mutants in the global regulator of virulence prfA and the alternative sigma factor sigB were unaffected and formed biofilms similar to wild type L. monocytogenes. |
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| Keywords: | Listeria monocytogenes Biofilm ami agrA Electron microscopy |
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