Lupeol reduces triglyceride and cholesterol synthesis in human hepatoma cells |
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Authors: | Mizuho Itoh Kazuyuki Hiwatashi Yukie Abe Fumiko Kimura Gen Toshima Junichiro Takahashi Hiroki Sasaki Keishi Hata |
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Institution: | 1. Skylight Biotech Inc., 100-4 Sunada, Iijima, Akita 010-0911, Japan;2. Akita Research Institute for Food & Brewing (ARIF), 4-26 Sanuki, Araya-machi, Akita 010-1623, Japan |
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Abstract: | Lupeol suppressed triglyceride and cholesterol secretion from HepG2-Lipo human hepatoma cells in a dose-dependent manner. Quantitative real-time RT-PCR analysis demonstrated that lupeol inhibited the expressions of sterol regulatory element-binding protein-1c and -2, fatty acid synthase, 3-hydroxy-3-methylglutaryl-Coenzyme A synthetase-1, and farnesyl-diphosphate farnesyl transferase-1, which are required for lipid synthesis in HepG2-Lipo cells. Furthermore, lupeol markedly inhibited apolipoproteinB-100 and microsomal triglyceride transfer protein in cells at the mRNA level. These results suggest that lupeol lowers lipid secretion from HepG2-Lipo cells by attenuating synthesis within the cells. |
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