Ultrasensitive Electrochemical Immunoassay for Avian Influenza Subtype H5 Using Nanocomposite |
| |
| Authors: | Zhixun Xie Jiaoling Huang Sisi Luo Zhiqin Xie Liji Xie Jiabo Liu Yaoshan Pang Xianwen Deng Qing Fan |
| |
| Affiliation: | Guangxi Key Laboratory of Animal Vaccines and Diagnostics, Guangxi Veterinary Research Institute, Nanning, Guangxi Province, China.; Boston University School of Medicine, United States of America, |
| |
| Abstract: | We report a novel electrochemical immunosensor that can sensitively detect avian influenza virus H5 subtype (AIV H5) captured by graphene oxide-H5-polychonal antibodies-bovine serum albumin (GO-PAb-BSA) nanocomposite. The graphene oxide (GO) carried H5-polychonal antibody (PAb) were used as signal amplification materials. Upon signal amplification, the immunosensor showed a 256-fold increase in detection sensitivity compared to the immunosensor without GO-PAb-BSA. We designed a PAb labeling GO strategy and signal amplification procedure that allow ultrasensitive and selective detection of AIV H5. The established method responded to 2−15 HA unit/50 µL H5, with a linear calibration range from 2−15 to 2−8 HA unit/50 µL. In summary, we demonstrated that the immunosenser has a high specificity and sensitivity for AIV H5, and the established assay could be potentially applied in the rapid detection of other pathogenic microorganisms. |
| |
| Keywords: | |
|
|
