Biological control of snow mould (Microdochium nivale) in winter cereals by Pseudomonas brassicacearum, MA250 |
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Authors: | Jens P Levenfors Thomas H Eberhard Jolanta J Levenfors Berndt Gerhardson Margareta Hökeberg |
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Institution: | (1) Department of Microbiology, SLU, P.O. Box 7025, Uppsala, 750 07, Sweden;(2) Maselaboratories AB, P.O. Box 148, Uppsala, 751 04, Sweden |
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Abstract: | Seed lots of winter wheat and rye, naturally infested with Microdochium nivale and Fusarium spp., were treated with an isolate of Pseudomonas, which was recovered from roots of Brassica napus. Seeds were treated with bacterial fermentate and dried before sowing or they were directly sprayed in the furrow-opener
at the moment of sowing. Besides field experiments, parallel climate chamber bioassays were performed to assess the effect
of bacterial treatment on snow mould caused by seed-borne M. nivale and Fusarium spp. The biocontrol effect was assessed by plant density counts and by measuring yield. Significant biocontrol activity,
measured by plant density counts, was detected both in field and climate chamber experiments sown with wheat. Biocontrol effect
after spray application at sowing was less pronounced, although a slight increase in plant density was observed. The cell
concentration required to obtain adequate biocontrol effect was 109 CFU per ml for the dose used. The bacterial isolate was identified by 16S rDNA sequencing and biochemical tests as a Pseudomonas brassicacearum strain. |
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Keywords: | Biocontrol Fusarium spp Gram-negative bacteria Field experiment Monographella nivalis Wheat |
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