Requirement of trypsin inhibitor for measurement of 3-hydroxy-3-methylglutaryl coenzyme A reductase in intestinal mucosa of rat |
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| Authors: | M Sugano T Ide H Okamatsu H Takahara |
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| Institution: | 1. Laboratory of Nutrition Chemistry, Kyushu University School of Agriculture, Fukuoka 812, Japan;2. Department of Animal Husbandry, Kyushu University School of Agriculture, Fukuoka 812, Japan |
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| Abstract: | A method was developed for the determination of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity in the microsomal fraction of crypt cells and villi of rat intestinal mucosa. Addition of trypsin inhibitor to homogenizing and incubation media at a proper concentration appeared inevitable for measurement of the activity of the villi fraction. The reductase in crypt cells was also slightly enhanced by the addition of the inhibitor. Using this technique, the enzyme activity in villi was found to be as active as the crypt cell fraction. Since other types of protease inhibitors were not necessarily effective, it was suggested that specific enzyme(s) inactivates the mucosal reductase in the course of measurement. |
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| Keywords: | LPO Lactoperoxidase PBS Phosphate buffered saline SDS Sodium dodecyl sulphate BSS Balanced salt solution EGTA Ethyleneglycol-bis-(β-amino-ethyl ether) N N′-tetra-acetic acid |
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