Identification and characterization of a UDP-GalNAc:GlcNAc{eta}-R {eta}->4-N-acetylgalactosaminyltransferase from cercariae of the schistosome Trichobilharzia ocellata. Catalysis of a key step in the synthesis of N,N'-diacetyllactosediamino (lacdiNAc)-type glycans期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

按检索

Identification and characterization of a UDP-GalNAc:GlcNAc{eta}-R {eta}->4-N-acetylgalactosaminyltransferase from cercariae of the schistosome Trichobilharzia ocellata. Catalysis of a key step in the synthesis of N,N'-diacetyllactosediamino (lacdiNAc)-type glycans

Authors:	Neeleman Alex P; der Knaap Wil PW van; den Eijnden Dirk Hvan

Institution:	Department of Medical Microbiology and Parasitology Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands ¹Department of Medical Chemistry, Faculty of Medicine, Vrije Universiteit Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands

Abstract:	Three different stages of the avian schistosome Trichobilharziaocellata appeared to contain a novel N-acetylgalac-tosaminyltransferaseactivity. To investigate its function in the biosynthesis ofschistosome glycoconjugates, the enzyme was partially purifiedfrom cercariae, a free-living stage of the parasite, by affinitychromatography on UDP-Sepharose. Acceptor specificity studiesshowed that the enzyme catalyses the transfer of N-acetylgalactosamine(GalNAc) from UDP-GalNAc to oligosaccharides, glyco-peptidesand glycoproteins carrying a terminally

Keywords:
本文献已被 Oxford 等数据库收录！