Reference genes for normalization of gene expression studies in human osteoarthritic articular cartilage |
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| Authors: | Manuel Pombo-Suarez Manuel Calaza Juan J Gomez-Reino Antonio Gonzalez |
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| Affiliation: | (1) Laboratorio de Investigacion 2 and Rheumatology Unit, Hospital Clinico Universitario de Santiago, Santiago de Compostela, Spain;(2) Department of Medicine, University of Santiago de Compostela, Santiago de Compostela, Spain |
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| Abstract: | Background Assessment of gene expression is an important component of osteoarthritis (OA) research, greatly improved by the development of quantitative real-time PCR (qPCR). This technique requires normalization for precise results, yet no suitable reference genes have been identified in human articular cartilage. We have examined ten well-known reference genes to determine the most adequate for this application. |
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