LXRs激动剂T0901317对成人骨骼肌细胞FAT／CD36基因mRNA表达的影响

目的：探讨肝核受体LXRs激动剂T0901317对正常人骨骼肌细胞中FAT／CD36基因mRNA表达的影响。方法：将原代培养的5例成人骨骼肌细胞分为用肝核受体LXRs激动剂39901317（1μmol／L）作用组、T0901317（0．5μmol／L）作用组和阴性对照组,作用24h后采用sYBR Green Ⅰ实时荧光定量PCR法检测各组成人骨骼肌细胞FAT／CD36基因mRNA表达水平,并用2^-^△△Ct方法进行比较分析。结果：①以浓度为1μmol／L的T0901317作用组和0．5μmol／L的T090131作用组和对照组样本的均数进行方差分析,差别有统计学意义（P〈0．01）。②浓度为1μmol／L的T0901317作用组和0．5μmol／L的T090131作用组成人骨骼肌细胞中FAT／CD36基因的mRNA表达分别是对照组的3．03倍和2．91倍。结论：肝核受体LXRs激动剂T0901317能够提高成人骨骼肌细胞中FAT／CD36基因mRNA的表达水平,提示30901317有加快骨骼肌细胞内脂肪酸的堆积作用,推测IXRs激动剂T0901317可能会增加糖尿病患者骨骼肌胰岛素抵抗的风险。

Effect of liver x receptors agonists T0901317 on expression of FAT/CD36 gene mRNA in adult human skeletal muscle cell

Aim： To investigate the liver X receptors agonists T0901317＇ s effect on expression of FAT/CD36 gene mRNA in adult human skeletal muscle cell. Methods： Myotubes from humans were exposed to different T0901317 concentrations（0, 0.5, and 1.0μmol/L） for 24 hours before experiments were performed. Then the expression of FAT/CD36 mRNA in skeletal muscle cell of each experimental group was detected by SYBR Green Ⅰ real-time quantitative polymerase chain reaction. The relative data were compared among groups by 2^-△△Ct method. Results： ①The Ct mean of control group,T0901317（0.5 μmol/L） group, T0901317（1 μmol/L） group were analyzed and there was significant differenee（P 〈 0.01）.②The expression of FAT/CD36 mRNA with liver X receptors agonists T0901317 in human skeletal muscle cell in the 39901317（0.5/.anol/L） group and T0901317（1/nnol/L） group were 2.91 times and 3.03 times than the control group. Conclusion： The expression of FAT/CD36 mRNA in human skeletal muscle cell afer the treatment of fiver X receptors agonists T0901317 is increased, so we may propose that T0901317 may increase the risk of resistance in adult human skeletal muscle.