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| 苦瓜种质遗传多样性的RAPD和ISSR分析 | |
| 引用本文： | 高山,林碧英,许端祥,傅睿清,林峰,林义章,潘东明.苦瓜种质遗传多样性的RAPD和ISSR分析[J].植物遗传资源学报,2010,11(1):78-83. |
| 作者姓名： | 高山 林碧英 许端祥 傅睿清 林峰 林义章 潘东明 |
| 作者单位： | 1. 福建省福州市蔬菜科学研究所,福州,350012;福建农林大学园艺学院,福州,350002 2. 福建省福州市蔬菜科学研究所,福州,350012 3. 福建农林大学园艺学院,福州,350002 |
| 摘 要： | 采用RAPD和ISSR分子标记技术对38份苦瓜种质进行遗传多样性分析。结果表明:10个RAPD和10个ISSR引物分别扩增出93条和81条带,多态性比率分别为50.54%和61.29%;RAPD和ISSR标记检测供试材料的遗传相似性系数(GS)范围,分别为0.287~1和0.221~1,ISSR(平均GS值0.672)检测多态性效果高于RAPD(平均GS值0.694)。RAPD标记聚类分析将供试种质分为3个类群6组,分类结果与苦瓜瓜瘤的表型分类比较相似;ISSR标记聚类分析将供试种质分为3个类群7组,ISSR标记划分类群与形态上以颜色分类比较接近。RAPD和ISSR标记的遗传相似性系数呈显著相关(r=0.550)。两个标记整合后聚类分析可检测到更大的遗传变异,结果与苦瓜的农艺性状分类和地理分布有一定的相关性。 |
| 关 键 词： | 苦瓜 种质 遗传多样性 RAPD ISSR |
Genetic Diversity of Bitter Gourd (Momordica charantia L.)based on RAPD and ISSR |
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| GAO Shan,LIN Bi-ying,XU Duan-xiang,FU Rui-qing,LIN Feng,LIN Yi-zhang,PAN Dong-ming.Genetic Diversity of Bitter Gourd (Momordica charantia L.)based on RAPD and ISSR[J].Journal of Plant Genetic Resources,2010,11(1):78-83. | |
| Authors: | GAO Shan LIN Bi-ying XU Duan-xiang FU Rui-qing LIN Feng LIN Yi-zhang PAN Dong-ming |
| Institution: | GAO Shan1,2,LIN Bi-ying1,XU Duan-xiang1,FU Rui-qing1,LIN Feng1,LIN Yi-zhang2,PAN Dong-ming (1 Fuzhou Institute of Vegetable Crops,Fuzhou 350012,2College of Horticulture,Fujian Agriculture , Forestry University,Fuzhou 350002) |
| Abstract: | Random amplified polymorphic DNA(RAPD)and inter-simple sequence repeat(ISSR)molecular markers were used to detect the genetic diversity among 38 bitter gourd(Momordica charantia L.).The results showed that 93 and 81 bands were obtained by RAPD and ISSR markers amplified through 10 selected primers respectively.The PPB(percentage of polymorphic bands)in ISSR detection(61.29%)was higher than that in RAPD(50.54%).The similarity coefficient ranging from 0.287 to 1 and from 0.221 to 1 respectively,and the ISSR(m... |
| Keywords: | RAPD ISSR Bitter gourd ( Momordica charantia L ) Germplasm Genetic diversity RAPD ISSR |
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