| 产纤维素酶黏细菌在生物转化的作用 |
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| 引用本文： | 王德韬,贺燕云,邱翔,马中良.产纤维素酶黏细菌在生物转化的作用[J].生物技术,2011,21(4). |
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| 作者姓名： | 王德韬 贺燕云 邱翔 马中良 |
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| 作者单位： | 上海大学生命科学学院,上海,200444 |
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| 基金项目： | 上海市高校选拔培养优秀青年教师科研专项基金项目 |
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| 摘 要： | 目的:预处理对木质纤维素降解的影响.方法:从土壤中分离筛选到高纤维素酶活的黏细菌菌株So ce sh1008.该菌具有CMC酶活(CMCase)及微晶纤维素酶活性.研究NaOH联合黏细菌降解盐蒿、稻草、棉花秸秆和甘蔗渣四种木质纤维素的情况.结果:碱(2％ NaOH) -黏细菌处理的方法优于黏细菌-碱的方法,其中降解棉花秸秆降解效果最明显,以5.0g木质纤维素为原料,其最终干重损失达2.1g,溶液中总糖含量和还原糖含量均值分别为12.8 mg/mL和0.93 mg/mL.酵母菌发酵产乙醇的研究结果表明,最佳发酵时间为47h,碱-黏细菌甘蔗渣降解液发酵效果最好,乙醇产出达6.0％.结论:黏细菌联合2％ NaOH能有效降解甘蔗渣,提高乙醇产量.
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| 关 键 词： | 黏细菌 木质纤维素 纤维素酶 生物转化 |
Myxobacterium Producing Cellulase in Conversion Lignocelluose to Ethanol |
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| WANG De-tao,HE Yan-yun,QIU Xiang,MA Zhong-liang.Myxobacterium Producing Cellulase in Conversion Lignocelluose to Ethanol[J].Biotechnology,2011,21(4). |
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| Authors: | WANG De-tao HE Yan-yun QIU Xiang MA Zhong-liang |
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| Institution: | WANG De-tao,HE Yan-yun,QIU Xiang,MA Zhong-liang(School of Life Sciences Shanghai University,Shanghai 200444,China) |
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| Abstract: | Objective:To investigate the effect of pretreatment methods on biomass.Method:Myxobacterium strains were isolated from soil by filter paper method,in which the strain So ce sh1008 with high cellulase activity was screened by Congo red.In this study,CMCase and microcrystalline cellulase activity of the strain were investigated.The hydrolysis of lignocellulose,namely artemisia stalk,rice straw,cotton stalk and bagasse via different pretreatments produced reducing sugar and total sugar.Result:The treatment wit... |
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| Keywords: | Myxobacterium lignocelluloses cellulase bioconversion |
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