|
|||||
|
|
| 甘肃肉羊新品种选育群GDF9基因外显子2多态性及其与产羔性状关联分析 | |
| 引用本文： | 朱爱文,马友记,陈国宏,孙寿永,李发弟.甘肃肉羊新品种选育群GDF9基因外显子2多态性及其与产羔性状关联分析[J].生物技术通报,2011(10). |
| 作者姓名： | 朱爱文 马友记 陈国宏 孙寿永 李发弟 |
| 作者单位： | 1. 甘肃农业大学动物科学技术学院,兰州730070;江苏畜牧兽医职业技术学院,泰州225300 2. 甘肃农业大学动物科学技术学院,兰州,730070 3. 扬州大学动物科学与技术学院,扬州,225009 |
| 基金项目： | 甘肃省自然科学基金项目(096RJZA008); 甘肃省农业生物技术项目(GNSW-2007-04); 国家科技支撑计划项目(2011BAD28B05) |
| 摘 要： | 根据GenBank发布的绵羊GDF9基因外显子2的序列设计4对引物,采用PCR-SSCP技术分析GDF9基因外显子2在甘肃内羊新品种选育群羊中的单核苷酸多态性,并与产羔性状进行关联分析.结果表明,GDF9基因的扩增片段在所检测的新品种群羊中存在PCR-SSCP多态性,检测到3种基因型(AA、AB和BB),而在32只无角陶赛特母羊群中只检测到AA和AB基因型.测序结果显示,GDF9基因编码区第978位碱基发生A→G突变,但没有导致氨基酸的改变；第994位碱基发生G→A突变,导致Ⅴ变成Ⅰ(缬氨酸→异亮氨酸).新品种选育群羊产羔数的最小二乘均值关系为AB＞ AA＞ BB,统计分析结果初步表明3种基因型之间差异不显著(P＞0.05).故该区域可能不是影响新品种群羊繁殖力的功能结构区城. |
| 关 键 词： | 肉羊 新品种群 繁殖力 生长分化因子9 (GDF9) PCR-SSCP |
Polymorphism of GDF9 Gene exon2 and Its Relationship with Litter Size Trait of Gansu Meat Sheep New Breed Population |
|
| Zhu Aiwen,Ma Youji,Chen Guohong,Sun Shouyong,Li Fadi.Polymorphism of GDF9 Gene exon2 and Its Relationship with Litter Size Trait of Gansu Meat Sheep New Breed Population[J].Biotechnology Bulletin,2011(10). | |
| Authors: | Zhu Aiwen Ma Youji Chen Guohong Sun Shouyong Li Fadi |
| Institution: | Zhu Aiwen1,2 Ma Youji1 Chen Guohong3 Sun Shouyong3 Li Fadi1(1College of Animal Science and Technology,Gansu Agriculture University,Lanzhou 730070,2Jiangsu Animal Husbandry and Veterinary College,Taizhou 225300,3College of Animal Science and Technology,Yangzhou University,Yangzhou 225009) |
| Abstract: | The GDF9 gene was studied as a candidate gene for the high prolificacy of sheep.Four pairs of primers were designed according to the sequence of exon2 of GDF9 gene published in GenBank.Single nucleotide polymorphisms(SNPs)of exon2 of GDF9 gene were detected in Gansu meat sheep new breed population and Poll Dorset by polymerase chain reaction(PCR)-single strand conformation polymorphism(SSCP).Polymorphism was detected in the amplified region,and three genotype(AA,AB and BB)in Gansu meat sheep new breed popul... |
| Keywords: | Meat sheep New breed population Prolificacy GDF9 PCR-SSCP |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |