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| 枯草芽孢杆菌TF26抗菌蛋白抑菌活性的初步研究 | |
| 引用本文： | 张淑梅,高娃,王玉霞,孟利强,李晶,赵晓宇,曹旭,沙长青. 枯草芽孢杆菌TF26抗菌蛋白抑菌活性的初步研究[J]. 生物技术, 2011, 21(4). DOI: 10.3969/j.issn.1004-311X.2011.04.109 |
| 作者姓名： | 张淑梅 高娃 王玉霞 孟利强 李晶 赵晓宇 曹旭 沙长青 |
| 作者单位： | 1. 东北农业大学园艺学院,黑龙江哈尔滨150030;黑龙江省科学院微生物研究所,黑龙江哈尔滨150010 2. 黑龙江省科学院微生物研究所,黑龙江哈尔滨150010;黑龙江省科学院高技术研究院,黑龙江哈尔滨150009 3. 黑龙江省科学院微生物研究所,黑龙江哈尔滨,150010 |
| 基金项目： | 哈尔滨市科技创新人才专项资金项目 |
| 摘 要： | 目的:研究枯草芽孢杆菌TF26抗菌蛋白的抑菌活性和生物稳定性,为菌株及抗菌蛋白的应用提供理论依据.方法:采用硫酸铵盐析方法提取抗菌蛋白,采用菌丝生长速率法检测其对13种植物病原真菌的抑菌活性,采用抑菌圈方法对其生物稳定性进行分析.结果:抗菌蛋白粗提物能够抑制13种植物病原真菌的生长,平皿抑制率为74.3％ ～91.3％,对葵花菌核病菌、番茄和黄瓜枯萎病菌、黄瓜菌核病菌和立枯病菌、水稻恶苗病菌和大豆根腐病菌抑制作用较强.抗菌蛋白在100℃以下,pH＜ 10范围内抑菌活性稳定,对紫外线照射不敏感,室温(20℃)和4℃储存150d抑菌活性稳定.结论:抗菌蛋白具有较强的热、酸碱、紫外和储存稳定性以及广谱的抑菌活性. |
| 关 键 词： | 枯草芽孢杆菌 抗菌蛋白 抑菌活性 稳定性 |
Study on Antagonistic Activity of Antifungal Protein from Bacillus subtilis TF26 against Plant Pathogenic Fungi |
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| ZHANG Shu-mei,,GAO Wa,WANG Yu-xia,MENG Li-qiang,LI Jing,ZHAO Xiao-yu,CAO Xu,SHA Chang-qing. Study on Antagonistic Activity of Antifungal Protein from Bacillus subtilis TF26 against Plant Pathogenic Fungi[J]. Biotechnology, 2011, 21(4). DOI: 10.3969/j.issn.1004-311X.2011.04.109 | |
| Authors: | ZHANG Shu-mei GAO Wa WANG Yu-xia MENG Li-qiang LI Jing ZHAO Xiao-yu CAO Xu SHA Chang-qing |
| Affiliation: | ZHANG Shu-mei1,2,GAO Wa3,WANG Yu-xia2,MENG Li-qiang2,LI Jing2,ZHAO Xiao-yu2,CAO Xu2,SHA Chang-qing2(1.College of Horticulture,Northeast Agricultural University,Harbin 150030,2.Institute of Microbiology,Heilongjiang Academy of Sciences,Harbin 150010,3.High Technique Council,Heilongiang Academy of Sciences,Harbin 150009,China) |
| Abstract: | Objective:To study antifungal activity and biological stability of antifungal protein from Bacillus subtilis TF26,and provide a theoretical basis for the application of the bacteria and antifungal protein.Method:The antifungal protein was isolated from fermentation broth using ammonium sulfate precipitation.The inhibition activity of antifungal protein against 13 plant pathogenic fungi were investigated by the method of mycelium growth inhibition.Moreover,the stability of antifungal protein was further anal... |
| Keywords: | Bacillus subtilis antifungal protein antagonistic activity stabilty |
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