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| 草莓果实MADS-box基因保守片段的克隆与序列分析 | |
| 引用本文： | 康兆茹,周厚成,赵霞,张敏,李凌.草莓果实MADS-box基因保守片段的克隆与序列分析[J].生物技术通报,2011(9). |
| 作者姓名： | 康兆茹 周厚成 赵霞 张敏 李凌 |
| 作者单位： | 1. 西南大学园艺园林学院,重庆400715;中国农业科学院郑州果树研究所,郑州450009 2. 中国农业科学院郑州果树研究所,郑州,450009 3. 西南大学园艺园林学院,重庆,400715 |
| 基金项目： | 河南省重点科技攻关项目(092102110038); 中国农业科学院科技经费项目(2009) |
| 摘 要： | 根据多种植物MADS-box基因保守区序列设计简并引物,应用PCR技术从草莓绿果中分高出33条MADS-box基因cDNA片段.序列分析表明,这些片段长度在137 - 146 bp之间,包含基因起始密码子.推导的氨基酸序列与已登录的草莓的MADS-box基因同源性超过87％.推导的氨基酸序列与已知的革莓和其他物种调控果实发育成熟的MADs-box基因以及拟南芥的MADS-box家族基因进行系统发育分析,可将这些基因片段分科归入拟南芥MADS-box基因不同亚家族中,证明草莓果实中存在各类MADS-box家族基因,克隆的部分片段可能参与调控果实发育和成熟软化的调节. |
| 关 键 词： | 革莓 MADS-box基因 RT-PCR 序列分析 |
Cloning and Sequences Analysis of MADS-box Genes Fragments from Strawberry( Fragaria ananassa Duch.)Fruit |
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| Kang Zhaoru,Zhou Houcheng,Zhao Xia,Zhang Min,Li Ling.Cloning and Sequences Analysis of MADS-box Genes Fragments from Strawberry( Fragaria ananassa Duch.)Fruit[J].Biotechnology Bulletin,2011(9). | |
| Authors: | Kang Zhaoru Zhou Houcheng Zhao Xia Zhang Min Li Ling |
| Institution: | Kang Zhaoru1,2 Zhou Houcheng 2 Zhao Xia2 Zhang Min1 Li Ling1(1College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,2Zhengzhou Fruit Institute,CAAS,Zhengzhou 450009) |
| Abstract: | According to the conservative region sequences of MADS-box family genes from various plants,we designed degenerate primers for obtaining 33 MADS-box gene fragments by RT-PCR from strawberry green fruit total RNA.Sequence analysis indicated the length of these fragments was between 137-146 bp,including start codon of genes,deduced amino acid sequences had a higher identity of over 87%with MADS-box genes of strawberry fruit.All these gene fragments were divided into the Arabidopsis MADS-box gene subfamily by ... |
| Keywords: | Fragaria ananassa MADS-box gene RT-PCR Sequence analysis |
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