| 一种根癌农杆菌介导的简单、快捷转化雪柑的新方法 |
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| 引用本文： | 王会全,曾黎辉,吴少华,徐海峰.一种根癌农杆菌介导的简单、快捷转化雪柑的新方法[J].生物技术通报,2011(9). |
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| 作者姓名： | 王会全 曾黎辉 吴少华 徐海峰 |
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| 作者单位： | 1. 福建农业职业技术学院园艺园林系,福州350119;福建农林大学园艺学院,福州350002
2. 福建农林大学园艺学院,福州,350002 |
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| 基金项目： | 福建省自然科学基金项目(B0510018); 教育部留学回国人员科研启动基金项目 |
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| 摘 要： | 以雪柑种子为外植体,以pC1301-PMI-LFY重组载体为转化载体,将蘸有农杆菌侵染液的接种针在种子胚的顶端分生组织部位刺2-3次,深度1 mm左右,当实生苗成活后移栽.通过PCR检测和PCR-Southern杂交检测得到了1株转基因植株,转化率为4.35％.针刺法简便快速,在雪柑上能够成功应用,为木本植物的遗传转化提供了新的思路.
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| 关 键 词： | 农杆菌介导 遗传转化 雪柑 LFY基因 针刺法 |
A Simple and Rapid Method for Transformation of "Xuegan" Using Agrobacterium tumefaciens |
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| Wang Huiquan,Zeng Lihui,Wu Shaohua,Xu Haifeng.A Simple and Rapid Method for Transformation of "Xuegan" Using Agrobacterium tumefaciens[J].Biotechnology Bulletin,2011(9). |
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| Authors: | Wang Huiquan Zeng Lihui Wu Shaohua Xu Haifeng |
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| Institution: | Wang Huiquan1,2 Zeng Lihui2 Wu Shaohua2 Xu Haifeng2(1Department of Horticulture and Garden,Fujian Vocational College of Agriculture,Fuzhou 350119,2Department of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002) |
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| Abstract: | To inoculate A.tumefaciens into the embryonic apical meristerm of the soaked seeds,a region on the seed suiface where a shoot would later emerge was pierced twice up to a depth of about 1 mm with a needle dipped in the A.tumefaciens inoculum.Germinated seedings were transplanted.One transgenic plantlet was obtained,which was confirmed by PCR assay and PCR-Southern blot.The transformation rate reached to 4.35%,and the feasibility of this method was proved,which could provid reference for the transgene of woo... |
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| Keywords: | Agrobacterium-mediated Genetic transformation Xuegan LFY gene Method of prickling |
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