Analysis of WT1 gene expression during mouse nephrogenesis in organ culture |
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Authors: | Herman Yeger Diane Forget Jennifer Alami Bryan R G Williams |
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Institution: | (1) Department of Pathology, The Hospital for Sick Children, 555 University Avenue, M5G 1X8 Toronto, Ontario, Canada;(2) Department of Cancer Biology, Cleveland Clinic Foundation Research Institute, 9500 Euclid Ave, 44195 Cleveland, Ohio |
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Abstract: | Summary The temporal and spatial expression patterns of the Wilms tumor gene, WT1, were studied during the organogenesis of the mouse
kidneyin vitro. In situ hybridization and immunocytochemistry localized cellular expression of WT1 in whole kidney organ cultures to the induced
metanephric mesenchyme and developing podocytes. Organ cultures were further characterized immunocytochemically with antibodies
that specifically labeled the different tubular epithelial components and supporting mesenchyme of the developing nephrons.
In organ cultures, the WT1 expression pattern could be visualized in induced metanephric mesenchyme and entire cell cohorts
of differentiating podocytes. Expression of WT1 and cell specific markers were retained in short-term monolayer cultures of
dissociated kidneys. The development of the metanephric kidneyin vitro involves a highly restricted temporal and spatial cellular expression pattern of WT1 which closely follows that observed
in tissue sections from gestational kidney isolated during organogenesis in the mouse. |
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Keywords: | nephrogenesis WT1 mouse organ culture in situ hybridization |
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