The production-influencing factors of extracellular polysaccharide (EPS) from a strain of lactic acid bacteria and EPS extraction |
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Authors: | Chen Ying Sun Liping Zeng Yong Wang Lei and An Liguo |
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Institution: | (1) College of Chemistry & Biology, Yantai University, Yantai, 264005, China;(2) College of Life Science, Shandong Normal University, Jinan, 250014, China |
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Abstract: | The influencing factors of extracellular polysaccharide (EPS) produced from a strain of lactic acid bacteria (LAB L15) were
studied by using the phenol-H2SO4 method. It was demonstrated that the strain produced EPS at the most amount when it was incubated for 40–48 h and when the
pH value was 4 under 30°C. Glucose was the most suitable carbon source for LAB-producing EPS. The rough EPS was obtained from
L15 culture after centrifugation, dialysis, deprotein, decoloration, and ethanol-precipitation. The sample was at least composed
of two polysaccharides that were completely different in molecular weight and the amount. The purified EPS was passed through
the SephadexG-200 column and it showed that it was a sample purified by thin layer chromatography.
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Translated from Microbiology, 2005, 32(4): 85–90 译自: 微生物学通报, 2005, 32(4): 85–90] |
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Keywords: | lactic acid bacteria extracellular polysaccharide (EPS) column chromatography thin layer chromatography |
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