Ginkgolide B production in cultured cells derived from Ginkgo biloba L. leaves |
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Authors: | Mee Hee Jeon Sang Hyun Sung Hoon Huh Young Choong Kim |
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Institution: | (1) College of Pharmacy, Seoul National University, San 56-1 Shillim-Dong, 151-742 Kwanak-Gu, Seoul, Korea |
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Abstract: | Callus cultures and cell suspension cultures derived from Ginkgo biloba L. leaves produced ginkgolidc B. In cell suspension cultures, the production reached a maximum by the 13th day of subculture and followed by a sharp decrease. The medium of Murashige and Skoog induced the highest ginkgolide B content in cultures while the medium of Schenk and Hildebrandt promoted cell growth. For the maximal production of ginkgolide B, cells were cultured in Murashige and Skoog medium modified to contain 1.0 mg/l of -naphthaleneacetic acid, 0.1 mg/1 of kinetin, 30 g/1 sucrose and 1.25 mM potassium phosphate with a molar ratio of ammonium to nitrate ions of 1 3.Abbreviations B5
Gamborg et al (1968) medium
- GKB
Ginkgolide B
- MS
Murashige and Skoog (1962) medium
- NAA
-naphthaleneacetic aicd
- SH
Schenk and Hildebrandt (1972) medium |
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Keywords: | |
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