基于响应面法的白蜡属花粉离体萌发培养基优化

基于Box-Behnken设计(Box-Behnken design, BBD)的响应面法, 对绒毛白蜡(Fraxinus velutina)、新疆小叶白蜡(F. sogdiana)和美国白蜡(F. americana)3个树种花粉的离体萌发培养基成分进行了研究。以花粉萌发率为响应指标, 建立了3种培养基成分(蔗糖、CaCl₂和H₃BO₃)与花粉萌发率间的响应关系模型。在此基础上, 通过无约束优化设计得到了3个树种花粉的最佳萌发条件。 结果表明, 蔗糖是影响花粉萌发的最主要因素, 当蔗糖浓度一定时, CaCl₂和H₃BO₃之间交互作用明显。同时还对响应面建模优化后得到的最佳萌发培养基进行了验证, 结果表明: 上述3种花粉的实际萌发率依次为58.33%、69.71%和59.42%, 均与优化得到的理论响应值相吻合, 同时也验证了基于BBD响应面模型进行花粉离体萌发条件优化方法的有效性。

Response Surface Methodology to Determine the Optimal Medium for Pollen Germination for Ash

We studied the pollen germination for three species of ash, Fraxinus velutina, F. sogdiana and F. americana. Response surface methodology (RSM) based on the Box-Behnken design (BBD) was used to construct a response surface model between the pollen germination rate and three ingredients of sucrose, CaCl₂ and H₃BO₃ for the culture medium. A non-constraint optimal design was adopted to obtain the optimal conditions of pollen germination for the three species on the basis of the obtained response surface model. The analysis of the model indicated that sucrose was the main influential factor for pollen germination. When the concentration of sucrose was at a certain threshold, the interaction between CaCl₂ and H₃BO₃ could be identified. The model was confirmed with pollen germination experiments corresponding to the three optimal media. The experimental results showed pollen germination rates of 58.33%, 69.71% and 59.42%, respectively. These proportions match theoretical response values well. They can also verify the effectiveness of the BBD-based response surface model and analysis technique in obtaining optimal concentrations of the culture medium components for pollen germination.