Dissociation of single cells from lung or kidney tissue with elastase |
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Authors: | Hugh J Phillips |
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Institution: | (1) Department of Physiology-Pharmacology, Creighton University School of Medicine, 68131 Omaha, Nebraska |
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Abstract: | Summary Experiments were conducted to determine the capacity of various enzyme preparations to dissociate single cells from guinea
pig lung tissue. The number of cells separated from tissue progressively increased as the concentration of crude trypsin was
increased from 25 to 250 mg per 100 ml. This action could be inhibited by soy bean trypsin inhibitor. Elastase, but not ethylenediaminetetraacetate
(disodium salt), crystalline trypsin, nor chymotrypsin, dissociated cells from lung tissues. Crude trypsin (Trypsin 1∶300)
was found to contain 3.0 Sachar units of elastase per mg. Elastase was also inhibited by soy bean trypsin inhibitor. Only
some collagenase preparations dissociated cells from lung tissue. Impure bacterial proteases dissociated lung cells. Our data
suggest that the term “trypsinization” to denote dissociation of cells from tissue with crude preparations of trypsin is misleading
and should be discontinued.
Partially supported bv Armour-Baldwin Laboratories and the National Institute of Health, Grant, AM 12919. |
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Keywords: | elastase lung tissue trypsin trypsinization |
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