| 棒状类细菌电击转化中多种条件对转化效率的影响 |
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| 引用本文： | 沈天翔,那淑敏,肖文中,贾盘兴.棒状类细菌电击转化中多种条件对转化效率的影响[J].生物工程学报,1995,11(3):245-249. |
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| 作者姓名： | 沈天翔 那淑敏 肖文中 贾盘兴 |
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| 作者单位： | 中国科学院微生物研究所 北京 100080 |
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| 基金项目： | 国家自然科学基金，科学院重点项目 |
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| 摘 要： | 以质粒PXZl0145电击转化不同棒状类细菌菌株，研究了影响电击转化效率的诸个因素，在含4%甘氨酸的培养基中生长至对数前期的菌体最适用于电击转化，当以同源DNA进行电击转化时，1．μgDNA中转化效率最高可达到8×1O6转化子，但用异源DNA时，转化效率要比前者低102～103倍。
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| 关 键 词： | 棒状类细菌，高压电击转化，转化效率 |
The Factors Affected Transformation Efficiency of Coryneform Bacteria by Electroporation |
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| Shen Tianxiang Na Shumin Xiao Wenzhong Jia Panxing.The Factors Affected Transformation Efficiency of Coryneform Bacteria by Electroporation[J].Chinese Journal of Biotechnology,1995,11(3):245-249. |
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| Authors: | Shen Tianxiang Na Shumin Xiao Wenzhong Jia Panxing |
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| Abstract: | High voltage electroporation was performed to transfer plasmid DNA of pXZl0145 into different kinds of corynebacteria strains. A number of factors that affected transformation efficiency were investigated. Cell grown in the presence of 4% glycine and harvested in the early exponential growth phase (OD600 was about 0. 2) was much easier to be transform. Transformation efficiency of up to 8X106 transformants per fig plasmid DNA with homologously derived DNA was obtained. If nonhorhologously derived DNA was used, transformation efficiency was 102 to 103 times lower than the former one. |
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| Keywords: | Coryneform bacteria electroporation transformation efficiency |
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