首页 | 本学科首页   官方微博 | 高级检索  
     


Line shape analyses for water 17O NMR quintet observed in a bacteriophage Pf1 solution at different temperatures
Authors:Mao Xi-an  Cui Wei  Gmeiner William H
Affiliation:Biochemistry Department, Wake Forest University School of Medicine, Medical Center Blvd, Winston-Salem, NC 27157-1016, USA. xmao@wfubmc.edu
Abstract:Partially resolved 17O NMR quintet was observed in a filamentous bacteriophage Pf1 solution at 70 degrees C with a quadrupole splitting approximately 100 Hz. As the temperature decreased, the resolution was reduced but the line shapes were still indicative of residual quadrupole splitting. Line shape analyses were performed using the quadrupolar relaxation theory for spin 5/2. The contribution to the residual quadrupole splitting from the electric field gradients stemming from the phage filaments, which were oriented in the magnet, was taken into account. As a result, the observed 17O spectra at different temperatures were simulated and the hydration number of the phage DNA was determined.
Keywords:
本文献已被 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号