| CRISPR-Cas9技术在细菌中的研究进展及应用 |
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| 引用本文： | 信欣,陈丽杰,薛闯. CRISPR-Cas9技术在细菌中的研究进展及应用[J]. 微生物学杂志, 2018, 0(6): 97-102 |
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| 作者姓名： | 信欣 陈丽杰 薛闯 |
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| 作者单位： | 大连理工大学 生命科学与技术学院，辽宁 大连 116023,大连理工大学 生命科学与技术学院，辽宁 大连 116023,大连理工大学 生命科学与技术学院，辽宁 大连 116023 |
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| 基金项目： | 国家自然科学基金项目(21576045，21376044)； 中央高校基本科研业务费专项资金资助项目（DUT16YQ103） |
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| 摘 要： | CRISPR (Clustered regularly interspaced short palindromic repeats)最早发现于绝大多数细菌和古细菌中,帮助其防御和抵抗噬菌体和外来质粒的入侵。近年来,随着对该系统结构及功能的逐步深入认识和研究,CRISPR-Cas9技术已经发展成为一种简易高效的基因组定点编辑手段,但是在使用过程中仍存在一些问题。本文重点介绍了CRISPR-Cas9系统功能和分类,在细菌基因编辑中的应用和问题以及应对措施。
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| 关 键 词： | 基因组编辑；CRISPR-Cas9；细菌；脱靶效应 |
Advance in and Application of CRISPR-Cas9 Technology in Bacteria |
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| XIN Xin,CHEN Li-jie and XUE Chuang. Advance in and Application of CRISPR-Cas9 Technology in Bacteria[J]. Journal of Microbiology, 2018, 0(6): 97-102 |
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| Authors: | XIN Xin CHEN Li-jie XUE Chuang |
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| Affiliation: | Schl. of Life Sci. & Biotech., Dalian Uni. of Technol., Dalian 116023,Schl. of Life Sci. & Biotech., Dalian Uni. of Technol., Dalian 116023 and Schl. of Life Sci. & Biotech., Dalian Uni. of Technol., Dalian 116023 |
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| Abstract: | CRISPR (Clustered regularly interspaced short palindromic repeats) was first found in most of bacteria and archabacteria, with the function to defend and resist the invasion from phage and foreign plasmid. In recent years, with the gradual understanding and research of the structure and function of the system, CRISPR-Cas9 technology has developed into a simple and efficient method of genomic editing. However, there are still some problems in the application process. This article focuses on the function and classification, the application and the problems and the solution in bacterial genome editing of CRISPR-Cas9 system. |
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| Keywords: | genome editing CRISPR-Cas9 bacteria off-target effects |
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