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A fast and inexpensive DNA extraction/purification protocol for brown macroalgae
Authors:GALICE HOARAU  JAMES A. COYER  WYTZE T. STAM  JEANINE L. OLSEN
Abstract:Here we describe a rapid method for extracting DNA from dried brown algae material using a microtitre plate system in conjunction with a milling instrument. The method allows the preparation of nuclear and organelle DNA of quality suitable for polymerase chain reaction amplification. It combines high throughput with low cost per sample: DNA from 192 samples can be extracted in c. 3 h for < €0.40 per sample, nearly tenfold cheaper than commercially available kits. Furthermore, by using microtitre plates, efficient storage and downstream processing is facilitated.
Keywords:brown algae  DNA extraction  heterokonts  high throughput  Phaeophyceae
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