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Characterization of simple sequence repeat markers derived in silico from Brassica rapa bacterial artificial chromosome sequences and their application in Brassica napus
Authors:ALISON E LING  JATINDER KAUR  BENJAMIN BURGESS  MELANIE HAND  CLARE J HOPKINS  XI LI  CHRISTOPHER G LOVE  MEGAN VARDY  MARZENA WALKIEWICZ  GERMAN SPANGENBERG  DAVID EDWARDS  JACQUELINE BATLEY
Abstract:A collaborative Brassica rapa genome sequencing project is currently in progress to aid the identification of agronomically important traits in Brassica species. As an initial stage, the ends of over 110 000 bacterial artificial chromosome clones were sequenced and mined for simple sequence repeats (SSRs). We present the characterization of 40 of these SSRs and their application in Brassica napus. The markers were screened against six Brassica species and Arabidopsis, and demonstrated reliable amplification, genome specificity, cross‐amplification and significant polymorphism. These SSRs will be useful for genetic analysis of Brassica germplasm.
Keywords:bacterial artificial chromosomes  Brassica  cross‐amplification  genome sequencing  genome specificity  simple sequence repeats
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