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Characterization of 12 polymorphic microsatellite loci for eastern chipmunks (Tamias striatus)
Authors:SARA J ANDERSON  JENNIFER A FIKE  GUHA DHARMARAJAN  OLIN E RHODES
Abstract:Eastern chipmunks are small forest rodents for which ecological data at multiple spatial scales exist, but no molecular markers have been developed that provide adequate resolution to examine genetic structure at fine scales in this species. Thus, we developed 12 variable microsatellite loci, which amplified in three multiplexed reactions, and screened them in 27 individuals from northern Indiana, USA. Observed heterozygosities across loci ranged from 0.26 to 0.91 and number of alleles per locus ranged from three to 10. We will use these markers along with ecological data to understand population dynamics of eastern chipmunks at small spatial scales.
Keywords:chipmunk  microsatellite  multiplex  Sciuridae  Tamias striatus
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