Structure and Chromosomal Localization of the Genomic Locus Encoding theKiz1LIM-Kinase Gene |
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| Authors: | Ora Bernard Vicki Burkitt Graham C. Webb Cynthia D. K. Bottema Jillian Nicholl Grant R. Sutherland Paul Matthew |
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| Affiliation: | aThe Walter and Eliza Hall Institute for Medical Research, P. O. The Royal Melbourne Hospital, Victoria, 3050, Australia;bDepartment of Animal Science, The University of Adelaide, Waite Campus, Glen Osmond, South Australia, 5064, Australia;cCentre for Medical Genetics, Women's and Children's Hospital, Adelaide, South Australia, 5006, Australia;dCentre for Animal Biotechnology, School of Veterinary Science, The University of Melbourne, Parkville, Victoria, 3052, Australia |
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| Abstract: | We have cloned and characterized the mouse gene encodingKiz1/Limk1,a new member of the zinc-finger LIM family that also has a kinase domain. The gene encompasses 25 kb of the mouse genome, and the organization of its 16 exons does not correlate with its functional domains. The promoter region ofKiz1/Limk1was identified by cloning a 1.06-kb genomic fragment upstream from the first ATG in a promotorless CAT vector. This construct was demonstrated to drive CAT expression in Jurkat cells. The promoter sequence lacks conventional TATA and CAAT motifs but contains consensus binding sequences for several transcriptional regulators implicated in control of transcription in many different cell types, including Sp1, Ets, and E2A. Analysis of the chromosomal localization ofKIZ1/LIMK1indicates that it lies on human chromosome 17 in the region 17q25 and on mouse Chromosome 5, band G2. |
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