Establishment and characterization of a new murine mammary tumor cell line,balb/c-MC |
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Authors: | Junji Morimoto Shunsuke Imai Yuzo Taniguchi Yoshihiko Tsubura Howard L Hosick |
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Institution: | (1) Department of Pathology, Nara Medical University, 840 Shijo-cho, Kashihara-shi, Nara-ken, Japan;(2) Department of Zoology, Washington State University, 99164 Pullman, Washington |
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Abstract: | Summary A new murine mammary tumor cell line (BALB/c-MC) was established from a spontaneous mammary tumor in a 17-mo.-old female mouse
of the low mammary cancer strain BALB/cHe. The cell line was derived from a papillary adenocarcinoma. In monolayer culture
the line exhibits a pavementlike arrangement of cells and forms “domes” or “hemicysts” as the cells become confluent. The
cell line rapidly forms tumors when transplanted into young syngeneic BALB/cHe mice. The subcutaneous injection of 106 cells resulted in the development of mammary tumors (typical papillary adenocarcinomas) in 33 of 37 (87% recipients within
2 to 3 mo. after injection. These mammary tumors also metastasize to lung 14 of 33 (42%) of recipients] during this time.
The number of chromosomes in this cell line is hyperdiploid (average of 43, range 39 to 44). |
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Keywords: | mammary tumor cells BALB/c mouse metastasis retrovirus |
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