| 热休克反应中小鼠心脏HSP70表达的免疫组织化学研究 |
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| 引用本文： | 郭玫,张顺利,张文学,魏纪东,张新胜,薛德明,徐存拴.热休克反应中小鼠心脏HSP70表达的免疫组织化学研究[J].动物学杂志,2001,36(2). |
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| 作者姓名： | 郭玫 张顺利 张文学 魏纪东 张新胜 薛德明 徐存拴 |
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| 作者单位： | 1. 河南师范大学生命科学学院
2. 河南师范大学生命科学学院新乡 453002 |
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| 摘 要： | 用免疫组织化学方法研究了小鼠心脏在不同温度（40、42、44、46℃）热休克处理后，各恢复期（2、4、8、12、24小时）HSP70 的表达。结果表明，（1）44、46℃热处理能诱导心肌细胞合成 HSP70，以46℃为多（P＜0.01），且于恢复期4～8小时为合成高峰（P＜0.01）；（2）阳性免疫反应定位于心肌细胞质中，核呈阴性反应。提示了心脏有较强的耐热能力。
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| 关 键 词： | 小白鼠 心脏 免疫组织化学 热休克蛋白70(HSP70) |
The Immunohistochemical Study of HSP70 Expression of the Mouse Heart in Heat Shock Response |
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| GUO Mei,Zhang Shunli,Zhang Wenxue,Wei Jidong,Zhang Xinsheng,XUE DeMing,XU CunShuan.The Immunohistochemical Study of HSP70 Expression of the Mouse Heart in Heat Shock Response[J].Chinese Journal of Zoology,2001,36(2). |
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| Authors: | GUO Mei Zhang Shunli Zhang Wenxue Wei Jidong Zhang Xinsheng XUE DeMing XU CunShuan |
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| Abstract: | The immunohistochemical method was used to analyze the expression of HSP70 in the heart of the mice which were treated at different temperature (40,42,44,46℃) for 30 minutes and had different recovery time (2,4,8,12,24 h). The results show that: (1) Cardiac muscle cells could synthesize HSP70 at 44℃ and 46℃, HSP70 was increased in 46℃ group (P＜0.01). There was peak value of HSP70 after 4～8 h recovery（P＜0.01）. (2) Positive immunoreactivity was localized in the cytoplasm while nucleus was negative. It suggests that the heart have strong thermotolerance in the heat shock. |
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