Effects of heterocyclic and tertiary permeant amines on the electron transfer in thylakoid membranes |
| |
Authors: | Opanasenko Vera Agafonov Alexey Demidova Raissa |
| |
Institution: | (1) Laboratory of Bioenergetics of Photosynthesis, Institute of Basic Biological Problems RAS, Pushchino, Moscow Region, 142290, Russia;(2) Laboratory of Mitochondrial Transport, Institute of Theoretical and Experimental Biophysics RAS, Pushchino, Moscow Region, 142290, Russia;(3) Laboratory of Photosynthetic Carbon Metabolism, Institute of Basic Biological Problems RAS, Pushchino, Moscow Region, 142290, Russia |
| |
Abstract: | The effect of low concentrations (up to 50 μM) of lipophilic permeant amines on the electron transfer in thylakoid membranes
of pea chloroplasts has been investigated. In the presence of heterocyclic amines (9-aminoacridine and neutral red), the electron
transfer, initiated from H2O to PS I acceptors, has been shown to be inhibited to a level amounting to less than 50% of control, this taking place for
both the basal (at alkaline pH) and the gramicidin-uncoupled transport (at pH 6.5–8.5). Under the same conditions, tertiary
amines (dibucaine, tetracaine) cause only a 10–15% inhibition of transport. With all the amines, the rate of electron transport
from H2O to DCBQ, PS II acceptor is decreased to 80–90% of control at pH above 8.0, but this effect is completely removed when pH
is lowered to 7.7–6.5. In the region of PS I, all the amines accelerate the basal transport, but do not influence the uncoupled
electron transfer. A conclusion has been drawn that, parallel with uncoupling, heterocyclic and tertiary amines also cause
an inhibition of PS II, appearing at alkaline pH values. Additionally, heterocyclic amines seem to brake electron flow at
the level of plastoquinone reduction.
This revised version was published online in June 2006 with corrections to the Cover Date. |
| |
Keywords: | chloroplasts electron transport local anesthetics permeant amines pH-indicators uncoupling |
本文献已被 PubMed SpringerLink 等数据库收录! |
|