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Effect of vitamin C depletion on age-related hearing loss in SMP30/GNL knockout mice |
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| Authors: | Akinori Kashio Akiko Amano Takashi Sakamoto Mitsuya Suzuki Tatsuya Yamasoba |
| Institution: | a Department of Otolaryngology and Head and Neck Surgery, University of Tokyo, Tokyo 113-8655, Japan b Aging Regulation, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan c Department of Biochemistry, Faculty of Pharmaceutical Sciences, Toho University, Chiba 274-8510, Japan |
| Abstract: | Using senescence marker protein 30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize vitamin C (VC), we examined whether modulating VC level affects age-related hearing loss (AHL). KO and wild-type (WT) C57BL/6 mice were given water containing 1.5 g/L VC VC(+)] or 37.5 mg/L VC VC(−)]. At 10 months of age, KO VC(−) mice showed significant reduction in VC level in the inner ear, plasma, and liver, increase in auditory brainstem response (ABR) thresholds, and decrease in the number of spiral ganglion cells compared to WT VC(−), WT VC(+), and KO VC(+) mice. There were no differences in VC level in the inner ear, ABR thresholds, or the number of spiral ganglion cells among WT VC(−), WT VC(+), and KO VC(+) mice. These findings suggest that VC depletion can accelerate AHL but that supplementing VC may not increase VC level in the inner ear or slow AHL in mice. |
| Keywords: | ABR auditory brainstem response AHL age-related hearing loss GNL gluconolactonase HPLC high-performance liquid chromatography KO knockout SGC spiral ganglion cell PBS phosphate buffered saline SMP30 senescence marker protein 30 SOD1 copper/zinc superoxide dismutase VC vitamin C WT wild-type |
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