The dibenzodioxocin lignin substructure is abundant in the inner part of the secondary wall in Norway spruce and silver birch xylem |
| |
| Authors: | Eija?M?Kukkola Sanna?Koutaniemi Eija?P?ll?nen Mikaela?Gustafsson Pirkko?Karhunen Taina?K?Lundell Pekka?Saranp?? Ilkka?Kilpel?inen Teemu?H?Teeri Email author" target="_blank">Kurt?V?FagerstedtEmail author |
| |
| Institution: | (1) Department of Biosciences, Division of Plant Physiology, Helsinki University, PO Box 56, 00014 Helsinki, Finland;(2) Department of Applied Biology, Helsinki University, PO Box 27, 00014 Helsinki, Finland;(3) Department of Chemistry, Laboratory of Organic Chemistry, Helsinki University, PO Box 55, 00014 Helsinki, Finland;(4) Department of Applied Chemistry and Microbiology, Helsinki University, PO Box 56, 00014 Helsinki, Finland;(5) Finnish Forest Research Institute, PO Box 18, 01301 Vantaa, Finland;(6) Department of Chemistry, University of Oulu, PO Box 3000, 90014 Oulu, Finland |
| |
| Abstract: | A specific condensed lignin substructure, dibenzodioxocin, was immunolocalized in differentiating cell walls of Norway spruce (Picea abies (L.) H. Karsten) and silver birch (Betula pendula Roth) xylem. A fluorescent probe, Alexa 488 was used as a marker on the dibenzodioxocin-specific secondary antibody. For the detection of this lignin substructure, 25- m cross-sections of xylem were viewed with a confocal laser-scanning microscope with fluorescein isothiocyanate fluorescence filters. In mature cells, fluorescence was detected in the S3 layer of the secondary wall in both tree species, but it was more intense in Norway spruce than in silver birch. In silver birch most of the signal was detected in vessel walls and less in fiber cell walls. In very young tracheids of Norway spruce and vessels and fibers of silver birch, where secondary cell wall layers were not yet formed, the presence of the dibenzodioxocin structure could not be shown.Abbreviation CLSM confocal laser-scanning fluorescence microscopy |
| |
| Keywords: | Betula Dibenzodioxocin Lignin substructure Picea Secondary wall |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
