| 酵母醇脱氢酶ADHI的纯化及动力学研究 |
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| 引用本文： | 廉德君,李林.酵母醇脱氢酶ADHI的纯化及动力学研究[J].生物化学与生物物理学报,1996,28(4):396-403. |
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| 作者姓名： | 廉德君 李林 |
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| 作者单位： | 中国科学院上海生物化学研究所，中国科学院生物物理研究所生物大分子国家重点实验室 |
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| 基金项目： | 国家自然科学基金,国家攀登计划项目资助 |
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| 摘 要： | 本文报道了啤酒酵母醇脱氢酶组成型同工酶ＡＤＨＩ的快速高效的纯化方法。通过活性蓝色染料柱亲和层析的方法将该酶纯化至电泳均一，收率达４７％，对该酶的产物抑制及端点抑制动力学研究结果支持Ｗｒａｔｔｅｎ，Ｃｌｅｌａｎｄ提出的序列有序机制。
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| 关 键 词： | 酵母 醇脱氢酶 同功酶 提纯 动力学 |
Purification And Kinetic Studies of Yeast Alcohol Dehydrogenase |
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| LIAN De-Jun,LI Lin and Xu Gen-Jun.Purification And Kinetic Studies of Yeast Alcohol Dehydrogenase[J].Acta Biochimica et Biophysica Sinica,1996,28(4):396-403. |
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| Authors: | LIAN De-Jun LI Lin and Xu Gen-Jun |
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| Abstract: | An efficient procedure for the purification of yeast alcohol dehydrogenase I(ADH I)was developed.By using Blue-Sepllarose 4B affinity chromatography,ADH I from yeast(S.cerevisiae)was purified 200-fold with an overall yield of47% to homogeneity as judged by sodium dodecy1 sulfate polyacrylamide gel electrophorsis and starch gel electrophoresis.Results of studies on the product and dead-end inhibition kinetics were in agreement with an orderde Bi Bi mechanism as proposed by Wratten and Cleland.Dioxane was found to be a competitive idhibitor of this enzyme.The complexity of the reaction mechanism of this enzyme is discussed. |
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| Keywords: | Yeast alcohol dehydrogenase Reaction mechanism Dead-end inhibition Affinity chromatography |
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