| 人参培养细胞中活性六糖的分离及结构鉴定 |
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| 引用本文： | 邓斌,郑光植.人参培养细胞中活性六糖的分离及结构鉴定[J].生物化学与生物物理学报,1996,28(6):600-605. |
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| 作者姓名： | 邓斌 郑光植 |
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| 作者单位： | [1]中国烟草育种研究中心 [2]中国科学院昆明植物研究所 |
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| 基金项目： | 国家自然科学基金云南省“八五’生物技术重点攻关资助 |
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| 摘 要： | 将用酸水解人参细胞方法得到的寡糖素，是一组聚合度由３－１２单糖组成的水溶性混合物，经活性碳柱，ｏｗｅｘ５０（Ｈ＾＋）柱，Ｂｉｏ－Ｇｅｌ Ｐ－２凝胶柱后由半制备型ＯＤＳ柱在ＨＰＬＣ分离到一个六糖，通过ＧＣ，ＧＣ－ＭＳ，ＦＡＢ－ＭＳ和＾１３４Ｃ－ＮＭＲ测定，推测出了这个
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| 关 键 词： | 人参 培养细胞 寡糖DP－6 六糖 |
The Isolation and Structure Characterization of the Active Oligosaccharide DP-6 from Cultured Panax ginseng Cells |
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| DENG Bing,ZHENG Guan-Zhi,SHEN Yu-Mao,LUO Jian-Ping and GAN Fang-Yuang.The Isolation and Structure Characterization of the Active Oligosaccharide DP-6 from Cultured Panax ginseng Cells[J].Acta Biochimica et Biophysica Sinica,1996,28(6):600-605. |
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| Authors: | DENG Bing ZHENG Guan-Zhi SHEN Yu-Mao LUO Jian-Ping and GAN Fang-Yuang |
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| Abstract: | The oligosaccharins isolated from acid-hydrolyzed cultured cells of Panax ginseng are a Mixtute of DP-3 to 12. The samples were passed through an active car bon column,a Dowex (H )column,and a Bio-Gel P-2 column to obtain oligosaccharides DP-6,7and 8.They were finally separated by HPLC and the DP6 oligosaccharide was obtained.Experiments showed that it could inerease the growth rate of many cultured Plant cells.Its structure was characterized by GC,GC-MS,FAB-MS and 13C-NMR measurements. |
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| Keywords: | Panax ginseng Cultured Cells Oligosaccharide DP-6 Structure |
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