Conserved and divergent patterns of expression of DAZL,VASA and OCT4 in the germ cells of the human fetal ovary and testis |
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| Authors: | Richard A Anderson Norma Fulton Gillian Cowan Shona Coutts Philippa TK Saunders |
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| Institution: | (1) Division of Reproductive and Developmental Sciences, Queen's Medical Research Institute, Edinburgh, EH16 4TJ, UK;(2) MRC Human Reproductive Sciences Unit, Queen's Medical Research Institute, Edinburgh, EH16 4TJ, UK |
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| Abstract: | Background Germ cells arise from a small group of cells that express markers of pluripotency including OCT4. In humans formation of gonadal
compartments (cords in testis, nests in ovary) takes place during the 1st trimester (6–8 weeks gestation). In the 2nd trimester
germ cells can enter meiotic prophase in females whereas in males this does not occur until puberty. We have used qRTPCR,
Westerns and immunohistochemical profiling to determine which of the germ cell subtypes in the human fetal gonads express
OCT4, DAZL and VASA, as these have been shown to play an essential role in germ cell maturation in mice. |
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