| 基于转录组测序探索创伤弧菌MO6-24/O小RNA |
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| 引用本文： | 周亚男,李宗城,张晓彤,胡成进,应晓敏,曹源. 基于转录组测序探索创伤弧菌MO6-24/O小RNA[J]. 生物技术通讯, 2019, 0(3): 354-359 |
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| 作者姓名： | 周亚男 李宗城 张晓彤 胡成进 应晓敏 曹源 |
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| 作者单位： | 潍坊医学院医学检验学系;解放军第九六〇医院实验诊断科;解放军总医院第五医学中心南院区肿瘤学研究室;军事医学研究院军事认知与脑科学研究所 |
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| 基金项目： | 国家自然科学基金(31100960) |
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| 摘 要： | 目的:基于转录组测序注释,探索创伤弧菌MO6-24/O小RNA(sRNA)。方法:在海洋培养基2216E中培养创伤弧菌MO6-24/O,收集从对数生长期到静止期不同时间点的细菌,提取细菌RNA,通过富集sRNA以及去除核糖体RNA进行建库和测序,然后将读段匹配到创伤弧菌MO6-24/O基因组并进行注释,最后进行验证。结果:发现59个顺式编码sRNA、102个反式编码sRNA,并进行了部分验证。结论:鉴定出创伤弧菌MO6-24/OsRNA。
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| 关 键 词： | 创伤弧菌 小RNA 转录组测序 |
Identification of Vibrio vulnificus MO6-24/O Small RNA Based on Transcriptome Sequencing |
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| ZHOU Ya-Nan,LI Zong-Cheng,ZHANG Xiao-Tong,HU Cheng-Jin,YING Xiao-Min,CAO Yuan. Identification of Vibrio vulnificus MO6-24/O Small RNA Based on Transcriptome Sequencing[J]. Letters in Biotechnology, 2019, 0(3): 354-359 |
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| Authors: | ZHOU Ya-Nan LI Zong-Cheng ZHANG Xiao-Tong HU Cheng-Jin YING Xiao-Min CAO Yuan |
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| Affiliation: | (Department of Medical Laboratory, Weifang Medical University, Weifang 261053;Department of Laboratory Medicine, 960th Hospital of the PLA Joint Logistics Force, Jinan 250031;Laboratory of Oncology, South Branch of General Hospital of PLA Fifth Medical Center, Beijing 100071;Institute of Military Cognition and Brain Sciences,Academy of Military Medical Sciences, Beijing 100850, China) |
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| Abstract: | ZHOU Ya-Nan;LI Zong-Cheng;ZHANG Xiao-Tong;HU Cheng-Jin;YING Xiao-Min;CAO Yuan(Department of Medical Laboratory, Weifang Medical University, Weifang 261053;Department of Laboratory Medicine, 960th Hospital of the PLA Joint Logistics Force, Jinan 250031;Laboratory of Oncology, South Branch of General Hospital of PLA Fifth Medical Center, Beijing 100071;Institute of Military Cognition and Brain Sciences,Academy of Military Medical Sciences, Beijing 100850, China) |
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