Effects of cultivation techniques and media on yields and morphology of the basidiomycete Armillaria mellea |
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Authors: | Göran Hansson Gunilla Seifert |
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Institution: | (1) Department of Applied Microbiology, Chemical Center, P.O. Box 124, S-22100 Lund, Sweden;(2) Division of Biological Sciences, National Research Council of Canada, 100 Sussex Drive, K1A OR6 Ottawa, Ontario, Canada |
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Abstract: | Summary The yield of cell mass and the morphology of Armillaria mellea, strain ATCC 11114, was studied using a variety of cultivation methods: solid media, standing liquid culture, shake flasks, tower reactors and impeller-stirred reactors. Two different media, malt extract broth and a glucose/asparagine/peptone-medium, and the corresponding agar media, were used. Yields were higher in the malt extract media than in the glucose media. Generally the highest yields were obtained on solid media while agitated cultures gave the lowest yields. Morphological characteristics such as pellet formation, adhesion to surfaces and pigment production were significantly affected by culture conditions. |
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