Adipocyte plasma membranes contain two Gi subtypes but are devoid of Go |
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Authors: | K D Hinsch W Rosenthal K Spicher T Binder H Gausepohl R Frank G Schultz H G Joost |
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Institution: | Institut für Pharmakologie, Freie Universit?t Berlin, Germany. |
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Abstract: | Antisera generated against synthetic peptides were used to identify G-protein alpha-subunits in plasma membranes from rat adipocytes. Applying the immunoblot technique, we detected two Gs alpha-subunits of 42 and 43 kDa, corresponding to the two cholera toxin substrates, and two Gi alpha-subunits of 40 and 41 kDa, corresponding to the two pertussis toxin substrates present in these membranes. The 40 kDa protein was tentatively identified as the Gi2 alpha-subunit. A serum specific for the Go alpha-subunit failed to detect any immunoreactive protein. Thus plasma membranes of adipocytes possess two forms of Gi but not Go. |
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