| PKCα translocation from mitochondria to nucleus is closely related to induction of apoptosis in gastric cancer cells |
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| 引用本文： | 吴乔,刘苏,丁亮,叶晓峰,苏文金.PKCα translocation from mitochondria to nucleus is closely related to induction of apoptosis in gastric cancer cells[J].中国科学：生命科学英文版,2002,45(3). |
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| 作者姓名： | 吴乔 刘苏 丁亮 叶晓峰 苏文金 |
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| 作者单位： | WU Qiao LIU Su DING LiangYE Xiaofeng & SU WenjinKey Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering，School of Life Sciences，Xiamen University，Xiamen 361005，China |
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| 基金项目： | This work was supported by the National Outstanding Youth Science Foundation of China (Grant No. 39825502)，National Natural Science Foundation of China (Grant Nos. 39880015 and 30170477). |
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| 摘 要： | PKCs have been implicated in the regulation of cellular differentiation, proliferation, apoptosis and signal transduction. It was demonstrated in this study that PKCa was located both at mitochondria and in cytosol in gastric cancer cell line BGC-823. Treatment of cells with 12-O-tetradecanoylphorbol-13-acetate (TPA) resulted in the translocation of PKCa from both mitochondria and cytosol to nucleus as clearly shown by laser-scanning-confocal microscopy, while the protein level of PKCa was not changed by TPA treatment as detected by Western blot. The results also revealed that TPA-induced translocation of PKCa was in close association with apoptosis induction, and such association was further affirmed by other experiments where various apoptotic stimuli and specific inhibitors of PKC were used. Taken together, these findings indicate that translocation of PKCa from both mitochondria and cytosol to nucleus in gastric cancer cell is accompanied by induction of apoptosis, and may imply a new mechanism of th
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