Polymers of random short oligonucleotides detect polymorphic loci in the human genome. |
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Authors: | G Vergnaud |
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Institution: | Laboratoire de Génétique Moléculaire, Centre d'Etudes du Bouchet, Vert-le-Petit, France. |
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Abstract: | Polymers of random 14 mer oligonucleotides are shown to detect discrete loci in the human genome. Eighteen different synthetic tandem repeats of random 14 base-pair units (STRs) have been generated and all of them turn out to detect polymorphic loci on southern blots of human DNA samples, presumably corresponding to a variable number of tandem repeats (VNTR). This finding suggests that minisatellites are a major component of the human genome and are strongly associated with the generation of genetic variability. In addition, it should open new strategies to make new polymorphic probes available. |
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