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Scribed transparency microplates mounted on a modified standard microplate |
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| Authors: | Brandon Huey-Ping Cheong Wei Seong Chua Oi Wah Liew Tuck Wah Ng |
| Affiliation: | 1. Laboratory for Optics and Applied Mechanics, Department of Mechanical & Aerospace Engineering, Monash University, Clayton, Victoria 3800, Australia;2. Cardiovascular Research Institute, Yong Loo Lin School of Medicine, National University of Singapore, National University Health System, Centre for Translational Medicine, Singapore 117599, Singapore |
| Abstract: | The immense cost effectiveness of using transparencies as analyte handling implements in microplate instrumentation offers the possibility of application even in resource-limited laboratories. In this work, a standard microplate was adapted to serve as the permanent base for disposable scribed transparencies. The approach is shown to ameliorate evaporation, which can affect assay accuracy when analytes need to be incubated for some time. It also offers assurance against fluorescence measurement errors due to the cross-talk of samples from adjacent wells. |
| Keywords: | Transparency Microplate Evaporation Cross-talk Green fluorescent protein |
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