Isolation, characterization and optimization of culture parameters for production of an alkaline protease isolated from Aspergillus tamarii |
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Authors: | Dayanandan Anandan William N Marmer Robert L Dudley |
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Institution: | (1) U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA |
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Abstract: | Aspergillus tamarii expresses an extracellular alkaline protease that we show to be effective in removing hair from cattle hide. Large quantities
of the enzyme will be required for the optimization of the enzymatic dehairing process so the growth conditions for maximum
protease expression by A. tamarii were optimized for both solid-state culture on wheat bran and for broth culture. Optimal protease expression occurred, for
both cultural media, at initial pH 9; the culture was incubated at 30 °C for 96 h using a 5% inoculum. The crude enzyme was
isolated, purified and characterized using MALDI TOF TOF. The alkaline protease was homologous to the alkaline protease expressed
by Aspergillus viridinutans.
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and does not imply recommendation or endorsement by the U.S. Department of Agriculture. |
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Keywords: | Aspergillus tamarii Broth culture Enzymatic dehairing Alkaline protease Solid-state fermentation |
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