Isolation,physical map and gene map of mitochondrial DNA from the cryptomonad Pyrenomonas salina |
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Authors: | Martina Maerz Peter Sitte |
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Institution: | (1) Lehrstuhl für Zellbiologie, Institut für Biologie II, Schänzlestr. 1, D-7800 Freiburg, Germany |
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Abstract: | Mitochondrial DNA (mtDNA) from the cryptomonad Pyrenomonas salina was isolated by CsCl-buoyant density centrifugation of whole-cell DNA in the presence of Hoechst dye 33258. mtDNA consists of circular molecules about 47 kb in size as estimated from restriction enzyme analysis. A physical map for six restriction enzymes (Bam HI, Bge I, Eco RI, Pst I, Sac I and Sac I) has been constructed. Genes coding for the small subunit of rRNA, cytochrome oxidase subunits I and II, and apocytochrome b were localized on this map using Southern blot hybridization with heterologous gene probes from Oenothera. Genes for 5S rRNA and NADH dehydrogenase subunit 5 are absent from P. salina mtDNA. The mitochondrial genome, being the first analysed to this extent in chromophytic algae, should be valuable for taxonomic and phylogenetic studies. |
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Keywords: | cryptomonads gene map mtDNA mitochondrial evolution Pyrenomonas salina restriction map |
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